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For the immunostaining of cultured PSCs, cells were fixed in 4% PFA for 15 min followed by a 15-min incubation in 0.5% Triton-X100

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[DOI] [PMC free article] [PubMed] [Google Scholar] 108.Prigge JR, Coppo L, Martin SS, Ogata F, Miller CG, Bruschwein MD, Orlicky DJ, Shearn CT, Kundert JA, Lytchier J, Herr AE, Mattsson A, Taylor MP, Gustafsson TN, Arner ESJ, Holmgren A, Schmidt EE

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In some patients, the immune response leads to viral destruction and downregulation of inflammation, while others generate a severe proinflammatory hypercytokinemia, multisystem inflammation, and shock, with some patients developing respiratory failure requiring mechanical ventilation [52] (Figure 1)