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The injections contain Riboflavin (Vitamin B2) which activates the metabolism of fat cells, Lecithin to break down and transport fat cells, and Bromelain Ananas Sativus (Pineapple) to break down fat and reduce inflammation

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After the cells reached 80% confluence, sample preparation for western blotting was performed using lysis buffer [50 mmol/l HEPES, 50 mmol/l NaCl, 1% (v/v) Triton X-100, 10% (v/v) glycerol, 1.5 mmol/l MgCl2, 1 mmol/l EDTA, 10 mmol/l sodium pyrophosphate, 1 mmol/l Na3VO4, 100 mmol/l NaF, 30 mmol/l 2-(p-nitrophenyl) phosphate, 1 mmol/l phenylmethylsulfonyl fluoride, 10 mg/ml leupeptin and 10 mg/ml aprotinin (pH 7.4)] as previously described (39)