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The benzyl alcohol doesnt kill existing bacteria instead, it slows or prevents new bacterial growth each time the rubber stopper is punctured

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Media was replaced every 2 days and monolayer integrity measured by TEER ( cm 2 , Millicell-ERS Voltohmmeter, Merck Millipore) every 7 days (16 h after media change)

Cumulative light exposure over many drawing sessions adds up even if each individual exposure seems brief