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doi: 10.1073/pnas.89.6.2394 112 PuD.ZhaoY.ChenJ.SunY.LvA.ZhuS.et al

Comparative example 1: 10-11Fmoc-Ala-Thr (pro-me-me) -OH, 21-22Fmoc-Ser-Ser (pro-me-me) -OH Cagrillinide preparation of peptide resin: the amino acids at the 10 th to the 11 th positions of the Cagrilintide sequence are used as pseudo-prolyl dipeptide Fmoc-Ala-Thr (pro-me-me) -OH, and the amino acids at the 21 st to the 22 th positions of the Cagrilintide sequence are used as pseudo-prolyl dipeptide Fmoc-Ser-Ser (pro-me-me) -OH Weighing Rink AmideAM resin 2.85g (Sub 0.35mmol/g) was added to the solid phase reactor, and 25mL DCM was added to swell the resin for 0.5 h