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Metabolic Pathway Investigation Research applications extend to metabolic process investigation: Glucose Metabolism Studies: Examination of GH effects on glucose uptake, insulin sensitivity, and hepatic glucose production Lipid Metabolism Research: Investigation of lipolysis activation, fatty acid oxidation, and lipid profile changes Protein Metabolism Studies: Research on protein synthesis rates, amino acid uptake, and nitrogen balance Energy Expenditure Investigation: Studies examining metabolic rate changes and substrate utilization shifts Metabolic Flexibility Research: Investigation of metabolic adaptation to altered GH/IGF-1 axis activity Laboratory protocols investigate metabolic effects using indirect calorimetry, glucose/insulin tolerance testing, and metabolic tracer studies in animal models

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The efficient delivery of PGC-1 siRNA to bladder cancer cells was achieved through the liposomal nanoparticles delivery system in this study, resulting in significant downregulation of the PGC-1 protein levels