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Materials and methods Primary human hepatocyte isolation Primary human hepatocytes (PHH) were prepared from nontumorous tissues obtained from freshly resected human livers of different donors (PHH Ctrl, n = 4) or explanted livers of WD patients (PHH WD, n = 2) via a two-step perfusion protocol as previously described [8]

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( Microsporum canis , there are 6 signaling pathways that have significant changes in Gene Ontology functional enrichment analysis after berberine treatment, including steroid biosynthesis, steroid hormone biosynthesis, Parkinsons disease, 2,4-Dichlorobenzoic acid (2,4-DCBA) degradation and biosynthesis of tropane, piperidine and isoquinoline alkaloids (Xiao et al., 2015)

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