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doi: 10.1146/annurev.immunol.18.1.275 119 LiuJWangNWuZGanYJiJHuangZet al

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Materials and methods Cell culture and treatments RTgill-W1 cell line was obtained from American Type Culture Collection (ATCC) and grown in Leibowitzs L-15 Glutamax (Gibco, Thermo Fisher, Waltman, MA, USA) supplemented with 10% FBS (EU standard, Biosera, Nuaille, France) and 1% penicillin/streptomycin (Lonza, Basel, Switzerland)

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Benfotiamine has been studied, mostly in laboratory settings and in animals, for its ability to mitigate the effects of high blood sugar on the nervous system, kidneys, and eyes (Balakumar 2010)