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Studying DILI Limitations with current techniques Poor in vitro to in vivo translation Standard 2D assays and miniaturized OOCs offer relatively low content insights Many advanced in vitro models are low throughput, limiting sample/ replicate number Short-lived in vitro assays cannot run chronic exposure assays Human metabolism is not adequately recapitulated in vitro or in vivo The human immune system is not adequately represented in vitro or in vivo Advancements with PhysioMimix Core Translate data from the lab to clinic using biomarker reporting Assess limitless endpoints to unlock the mechanism of DILI Generate full dose-response curves for multiple compounds/ plate Long-term cultures facilitate prolonged repeat dosing for weeks Correlate toxic phase I & II metabolite production with cell health Human tissue resident immune component captures inflammation-based adverse effects Standard human DILI cell culture timeline Standard cross-species DILI cell culture timeline Limitless endpoint measurements View the drop-downs for standard DILI assay longitudinal and endpoint measurements from recovered media and liver tissues