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Humidity in the storage environment, condensation from temperature changes, or a compromised vial seal can introduce enough water to begin degradation pathways even in the powder form

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The inoculated cells were incubated at 37C without shaking, and aliquots (100 L) were withdrawn at specific time intervals (0, 4, 8, and 12 hours) and spread the cells on plain LB agar plates with an appropriate dilution in case

Third, given their reliance on lipid metabolism, B1 and MZB cells are extremely susceptible to lipid peroxidation and subsequent ferroptosis secondary to ROS accumulation 142