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The total number of actin enriched spines adjacent to VGLUT1 or Synapsin were counted and converted to a percentage as the percent correlation of treatment-induced spines to presynaptic markers is a strong indicator of ability to transmit excitatory signals

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Moreover, through the reduction of ROS formation, stabilization of mitochondrial membrane potential, restriction of mPTP opening, regulation of caspase-3 activity, and eventually suppression of cardiomyocyte apoptosis, VA protects H9c2 cells against H/R-induced damage [32]

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