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In vitro modulation of T-cell surface molecules by iron

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The frozen tissues were weighed, and then 1.5 mL 80% methanol solution containing 4 g/mL 2-chloro-L-phenylalanine was homogenized for 2 min

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In vitro perfusion studies, using isolated proximal convoluted tubules and in vitro studies using OK cells, showed that IGF-1 directly increases phosphate reabsorption via increase of protein levels of Na-Pi2a at the plasma membrane, which could be completely blocked by an anti-IGF-1R antibody [51,52,53]