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90% of physicians report concern about patients self-directing peptide use without oversight

After washing with PBST buffer at room temperature, the membrane was incubated with horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG secondary antibody (1:10,000, BA1054, Boster, Wuhan, China) at room temperature for 1 h

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Further, NAC and diNAC content was evaluated via HPLC to determine the extent of dimerization