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Keep in mind that once the vial is opened, sterile water cannot be stored after that, as it must be used immediately

Reusing Syringes: A new, sterile syringe should be used for every single draw from the vial

I was very calm but sobbing and pretty much begging for help over the phone and mentioned I would need to seek legal advice as this is affecting my day to day life

These sequences were then run through an R script (Supplementary Materials, bash_DADA2 + IDTAXA_workflow_Illumina-PairedEnd_V1V3.R) which trimmed reverse reads to 260 bp, removed forward reads exceeding a maximum expected error of three, and truncated reverse reads exceeding a maximum expected error of six

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