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Selective ghrelin receptor modulation by Ipamorelin

RNA extraction and reverse transcription-quantitative polymerase chain reaction (RT-qPCR) We isolated RNA from zebrafish skeletal muscle using the NucleoSpin RNA Midi kit (MACHEREYNAGEL, US), and performed reverse transcription-quantitative PCR (RT-qPCR) according to the protocol as described in our paper 28