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Upon binding to IP 3 , IP 3 receptor type 1 (IP 3 R1) releases Ca 2+ from endoplasmic reticulum (ER), thus triggers the transcription of gluconeogenic genes

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10.1111/dom.12591 64 NelsonM.BhandariN.WenerJ

The protein samples (12 L) and Spectra Multicolor Broad Range Protein Ladder (7.5 L) with 10260 kDa range (Thermo Fisher Scientific) were loaded onto the gel, and electrophoresis was run at 200 V for 22 min in 1 Bolt MES SDS Running Buffer (Invitrogen)