cDNA was prepared from total RNA and used for gene expression by real-time quantitative RTPCR (qPCR) as described [53] using TaqMan Gene Expression Assays (ThermoFischer Scientific) specific for human GCLC (Hs00155249_m1), OCT4 (Hs00999632_g1), and ACTB (Hs01060665_g1)
Therefore, considering the widespread use of these medications, it is crucial to elucidate the potential role of GLP-1RA use in the development of thyroid cancer
However, more dilute concentrations may require less bacteriostatic water
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