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doi:10.2174/187152009789056895 Zhao GS, Wang XB (2006) Advance in antitumor agents targeting glutathione-S-transferase

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Cell fractionation and membrane preparation RBL cells (5 X 10' cells/ml) were sonicated 3 X IO s in ice-cold sonication buffer (20 mM HEPES, 1 mM MgCI,, 1 mM EDTA, 1 mM DTT, 1 mM Na,VO,, and 10 mM NaF protease inhibitors (41))

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Together, these experiments confirmed the strong tonic inhibitory role of somatostatin on glucagon and insulin secretion as previously demonstrated [12]