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Macrophages acquire exogenous lipids through phagocytosis, micropinocytosis, or recognition by scavenger receptors such as CD36 to obtain lipid droplets (146)

After seeding the cells in a 24-well plate for 24 h, they were transfected with either the control lentivirus (Lv-NC), PPARa over-expression lentivirus (Lv -PPARa , GV341, Ubi-MCS-3FLAG-SV40-puromycin), or PPARa shRNA lentivirus (Lv -PPARa RNAi, GV112, hU6-MCS-CMV-Puromycin) at a multiplicity of infection (MOI) of 50, the details of Lv -PPARa RNAi could be found in Table 1

A crystal structure of 34b in complex with the yeast 20S proteasome was also obtained to validate the molecular design