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The plates were read at 450 nm and concentrations obtained in pg./ml from a standard curve using a 4-parameter curve fit with blank control subtracted absorbance values and a 15% CV cut-off

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Lancet Oncol 18(10):14111422 Franz DN et al (2013) Efficacy and safety of everolimus for subependymal giant cell astrocytomas associated with tuberous sclerosis complex (EXIST-1): a multicentre, randomised, placebo-controlled phase 3 trial

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