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Magnetic cell sorting (MACS) The spleen (SP) of C57BL/6 mice was obtained aseptically to prepare a single-cell suspension, and the red blood cells were removed with red blood cell lysate (Beijing Tiangen Biochemical Technology Co., LTD

When appropriate, compounded medications may be incorporated into the program along with continued monitoring and adjustments over time

This age-related decline is one of the most frequently cited data points in copper-peptide research literature, and its a primary reason GHK-Cu is studied within the broader context of cellular aging and peptides for longevity research rather than treated purely as a wound-healing or dermatology compound