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Lentivirus production The following plasmids were used for lentivirus production: psPAX2 packaging plasmid (Addgene, #12260), pMD2-G VSV-G envelope plasmid (Addgene, #12259), Blast-Cas9 plasmid, pKLV2-U6gRNA5(gGFP)-PGKBFP2AGFP-W reporter plasmid (Addgene, #67980), and custom pooled CRISPR libraries

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Selenium mitigated aflatoxin B 1 -induced cardiotoxicity with potential regulation of 4 selenoproteins and ferroptosis signaling in chicks