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Autophagy 12, 14251428

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-galactosidase activity was detected using X-Gal staining solution (1 mg ml 1 X-gal, Thermo Scientific, 5 mM K 3 (Fe(CN) 6 ) and 5 mM K 4 (Fe(CN) 6 )) diluted in 1 mM MgCl 2 /PBS (pH 5.56) for 818 h at 37 C