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For the isolation of PBMCs, blood from the same donor was pooled and diluted with an equal volume of PBMC isolation buffer containing 0.1% bovine serum albumin (Carl Roth, Karlsruhe, Germany) and 2 mM ethylenediaminetetraacetic acid (EDTA) (Sigma-Aldrich) in phosphate buffered saline (PBS) without calcium and magnesium (Lonza, Cologne, Germany)

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And when you look at my coronary arteries on a heart scan, theres no atherosclerosis at all and at 71 years of age, thats rare

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Another electrolyte serving in the afternoon