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CHIR99021 was removed after 72 h and DE cells were cultured in RPMI/B27 + Insulin for 24 h ( For growth factor Definitive Endoderm (DE) differentiation, human iPSCs cultured in 6-well dishes to 60% confluence were washed sequentially with DPBS / and DMEM/FI2 medium (Thermo Fisher Scientific)

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Severe thiamin deficiency, which is rare in industrialized nations, except in patients with chronic alcoholism, HIV-AIDS, or gastrointestinal conditions that impair vitamin absorption (23), results in the condition called beriberi, of which there are many forms that involve neurological symptoms

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Int J Mol Sci 24:3393 Shulhai A-M, Rotondo R, Petraroli M et al (2024) The role of nutrition on thyroid function