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However, during pregnancy, E3 level increases about 1,000-fold, which accounts for over 90% of the total circulating estrogen under normal physiological conditions [50, 51]

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C/1000 = (OD2 OD1) / 13,600 E1 5/2 13,600: Molar extinction coefficient of yellow color formed during the interaction of GSH and 5,5-dithio-bis-(2-nitrobenzoic acid) (DTNB)