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16:0) covalently attached to the side chain amine of Lys10 in peptide 20 through a -carboxylate spacer and the Glu-2OEG linker, and C18 fatty diacid moiety that was acylated on Lys20 in tirzepatide were modeled with the CHARMM CGenFF small-molecule force field 66 , program version 1.0.0