Briefly, mice were euthanized by CO2 inhalation and cervical dislocation as aforementioned, before livers were immediately removed, minced and placed in ~10-fold volume of ice-cold mitochondria isolation medium [250 mM sucrose, 10 mM Tris/hydrogen chloride (HCl), 1 mM EGTA and 1% fatty acid/endotoxin-free BSA
While most insurance plans provide coverage for this groundbreaking treatment, individual policies may vary
If such were to happen, the door to expand access to coverage remains possible in the future
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