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The sll1545 :Sm r /Sp r cassette was generated by replacing the Km r DNA cassette by the Sm r /Sp r DNA cassette from pFC1 plasmid ( The resulting deletion cassettes sll1545 :Km r , slr0236 :Km r and sll1545 :Sm r /Sp r were verified by PCR and nucleotide sequencing (Mix2Seq Kit, Eurofins Genomics) before and after transformation ( Synechocystis WT cells (to generate the sll1545 :Km r and slr0236 :Km r mutants) or to the slr0236 :Km r mutant (to generate the sll1545-slr0236 mutant)

Therefore, bacteriostatic water is an essential component for reconstitution of peptides from dried form into liquid form for use in research laboratories

Int J Androl 2012

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