When you take a high dose of traditional vitamin C all at once, your intestinal transporters can become overwhelmed, leading to decreased absorption and potential digestive discomfort
The main reason might be that high temperature seriously suppresses the activities of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) large subunit (RBCL), Rubisco small subunit (RBCS) and other related enzymes 12 , and leads to impairment of chlorophyll biosynthesis 13
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However, the observations are consistent with the earlier suggested role of 5-oxoproline as a relatively inert with a role as an osmoprotection
After treatments, cells were incubated with MTT for 4 h, then the supernatant was removed, and the formazan crystals were dissolved in 200 L dimethyl sulfoxide (DMSO), the optical density was measured at 570 nm
