This amount roughly corresponds to the daily turnover in healthy people and seems a reasonable dosage free of side effects, either for a preventive or therapeutic approach
The flow rate was expressed in blood perfusion units (BPU)
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Biomedicines 10:945 Saxena M, Burke A, Pavlick A, Blazquez A, Gimenez G, Meseck M, Donovan M, Rodriguez D, Castillo-Martin M et al (2022) Abstract CT108: immunogenicity of poly-ICLC matured dendritic cells as an adjuvant for NY-ESO-1 and Melan-A-MART-1 peptide vaccination compared to Montanide ISA-51 VG, in study subjects with melanoma in complete clinical remission but at high risk of disease recurrence
The animals were anesthetized with urethane (100 mg/kg i.p.) and transcardially perfused with 100 ml of saline and heparin (200 l/L), followed by 200 ml of 4% paraformaldehyde in 0.1 M PBS (pH 7.4)