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Mass spectrometry only measures for what we are looking for, whereas ELISA can also cover metabolites of the toxins we are looking for

Studies demonstrate that LPS activates adipose tissue macrophages via TLR4 receptors, promoting the release of proinflammatory cytokines such as TNF-, which in turn induces excessive lipolysis in adipocytes, generating large quantities of free fatty acids (FFAs) ( 3.5 Hypothalamic-pituitary-ovarian axis disruption Disruption of gut microbiota leading to systemic LPS translocation not only impairs peripheral organ function but also exacerbates PCOS pathology by interfering with the neuroendocrine regulation of the HPO axis ( 4 Gut microbiota characteristics in PCOS Recent studies have shown distinct differences in gut microbial composition between PCOS and healthy controls

First, since the clinical parameters were obtained from a public database, some clinical data were not available such as chemotherapy, smoking and infection with Helicobacter pylori, and therefore, a comprehensive analysis was not performed

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