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Its pathogenesis is complex and multifactorial, with oxidative stress recognized as a central mechanism that interacts with metabolic dysregulation, mitochondrial dysfunction, and chronic inflammation

Advanced experimental models integrating vascular remodeling, BBB integrity, and neurovascular interactions are essential to accurately simulate the dynamics of chronic disease

After reconstituting with Bacteriostatic Water, the solution must be stored in a refrigerator at 2-8C and should never be frozen

Immunohistochemistry The fixed tissues were processed using routine histological procedures and mounted on positively charged slides

Altered transporter protein levels and inflammation markers, accompanied by characteristics of vessel degeneration but also of angiogenesis, comprise the most important alterations found at the BBB of PD patients (Fig