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882 In a classic example, Thorson and coworkers generated a large library of vancomycin ( 3 ) derivatives

Trx becomes reduced through an intermediate selenenylsulfide between Cys-32 of Trx and Sec-498 of TrxR, and Tyr-116 acting by (i) facilitating selenenylsulfide reduction in the reductive half-reaction through resonance effects and, possibly, (ii) facilitating release of the intermediate selenenylsulfide with Trx by its interaction with the selenenylsulfide between Cys-497 and Sec-498