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Binding to HLA-C*06:02 was verified using the NetMHCpan - 4.1 server ( Generation of plasmid-encoded peptides for V3S1/V13S1-TCR hybridoma stimulation For expression of short candidate peptides, forward and reverse oligonucleotides (Biomers) carrying a 5-CACCATG overhang and a stop codon in 3-position of the target sequence ( Supplementary Table S2 ) were annealed and ligated into pcDNA3.1D/V5-His-TOPO using pcDNA3.1 Directional TOPO Expression Kit (Invitrogen, Carlsbad, USA) according to manufacturers instructions as described ( Culture conditions and stimulation of the CD8 + V3S1/V13S1-TCR reporter T-hybridoma cell line Generation and culture conditions of the V3S1/V13S1-TCR CD8 + reporter T hybridoma from the paired -TCR chains rearranging the V3S1 and the V13S1 gene segments of a lesional psoriatic CD8 + T-cell clone have been described ( To determine the antigenicity of peptide antigens for the V3S1/V13S1-TCR hybridoma, COS-7 cells were cotransfected with HLA-C*06:02- and peptide-encoding plasmids (250 ng each) using FuGENE HD reagent (Promega)

Ehrenstein, G

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Key molecular features include the F23 Cagri residue anchoring the peptide at the receptor transmembrane (TM) bundle level and the micelle, an E14-R17 intramolecular salt bridge enhancing helical stability, and C-terminal P37 Cagri interaction with the receptor ECD