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The assay plate was incubated for 3 h at 37 C in 5% CO 2 and allowed to equilibrate at room temperature for 5 min after which 30 L SteadyLite Plus (PerkinElmer, 6066759) was added to each well

Supplement with either glutathione tripeptide (500 mg daily) or the precursor of glutathione, N-acetylcysteine (500600 mg daily)

Folic acid: the only universally recommended preconception supplement Folic acid is the one supplement you should be taking if you're planning a pregnancy, regardless of your age

The research was supported by the National Institutes of Health

The role of oxidative stress in skeletal muscle injury and regeneration: focus on antioxidant enzymes