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For Ozempic (multi-dose pen with detachable needles): Remove the pen from refrigeration 1530 minutes before injection to reduce discomfort Wash hands thoroughly with soap and water Attach a new needle for each injection Perform a flow check before first use of each new pen only (not before every dose) Clean the injection site with an alcohol swab and allow to dry completely For Wegovy (single-dose pen with built-in needle): Remove the pen from refrigeration 1530 minutes before injection Wash hands thoroughly Do not prime the pen (it comes ready to use) Clean the injection site with an alcohol swab and allow to dry completely Injection technique for both products: Pinch a fold of skin gently between thumb and forefinger to elevate subcutaneous tissue away from underlying muscle

Single-cell RNA sequencing of adult intestinal crypts and data analysis