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The analysis revealed that Day 30 represents the peak of functional maturity, marked by the highest expression of key-metabolizing enzymes, such as cytochrome P450 3A4 (CYP3A4), along with cholangiocyte-specific proteins and hepatic metabolic enzymes

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Consistently, our molecular dynamic (MD) simulations found that the ECD interacted with the TMD intimately, such that the N-terminal -helix stably inserted to the TMD core (Supplementary Fig