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A3 An amount of 50 mL of the biomass suspension (step A1) + 40 mL DMEM +10 mL sterile hydroalcoholic solution (90% H 2 0/10% EtOH) (total volume 100 mL, the reference preparation was without the matrix)

Duriancik DM, Lackey DE, Hoag KA

Western blot results showed that GHK-Cu significantly upregulated SIRT1 expression in the colon tissues of DSS-induced mice and MCECs in the co-culture system

In preclinical and in vitro studies, GHK and GHK-Cu have been investigated in the context of copper-coordination chemistry, extracellular-matrix remodeling, collagen and metalloproteinase regulation, and antioxidant, anti-inflammatory, and gene-expression signaling

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