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The tissues were rinsed with PBS and incubated with Alexa Fluor488 chicken anti-rabbit (1:1000, Life Technologies) for one hour at 37 C and then rinsed with PBS and incubated with Alexa Fluor488 goat anti-chicken antibody (1:1000, Life Technologies) and Alexa Fluor568 goat anti-mouse antibody (1:1000, Life Technologies) for another hour at 37 C

(2023) Bunyavanich S et al

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H., Hsu, F

Real-time quantitative polymerase chain reaction (qRT-PCR), cDNA synthesis and RNA extraction cDNA was synthesized using the RevertAid First Strand cDNA Synthesis Kit (Thermo Fisher Scientific, Waltham, MA, USA)