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Nat Med 32 , 342352 (2026)

Standard laboratory protocols are summarized below researchers should validate against their own internal SOPs

POMC neuron ablation was performed on 5-week-old iDTR:: Pomc -Cre mice by intracerebroventricular (i.c.v.) infusion of 2.5 ng of diphtheria toxin (DT) dissolved in 2.0 L of saline, followed by a 2-week period to ensure complete cell-lineage ablation [11-13]

Research vials usually come in 2 mg, 5 mg, or 10 mg sizes and are mixed with bacteriostatic water before use

Peptide therapy involves the use of short chains of amino acids (peptides) that signal cells to perform specific biological functions