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It's yes, always, 28C, from first puncture to final draw or day 28

Aseptic technique per draw: Pharmaceutical handling documentation and community sources consistently describe the same sequence: The rubber stopper surface is wiped with a fresh alcohol swab before each puncture The alcohol is allowed to air-dry (community sources cite 10-30 seconds) A fresh, sterile needle is used for each draw The needle is inserted at the documented angle through the stopper center The required volume is drawn and the needle is removed Needle reuse