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Cellular autophagy and cell death In DPN, hyperglycemia impairs autophagy by inhibiting key autophagy-related proteins, resulting in the accumulation of dysfunctional mitochondria and protein aggregates

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Therefore, one might argue that its inhibition may contribute to peritoneal fibrosis prevention and preservation of the peritoneal membrane.39 Peroxisome proliferator-activated receptor- (PPAR-) agonists, thiazolidinediones (TZD) are oral anti-diabetic agents thought to act through increased GLUT1 and GLUT 4 cell surface expression, leading to increased glucose uptake and reduced serum glucose levels

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Cell pellets, obtained by 8000 x g centrifugation, were flash frozen in liquid nitrogen and stored at 20 C