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Because of this, oral vitamin B12 is considered to be safe even at high doses

Cell and tissue research , 377 (2), 153159

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Finally, after washing twice with PBS buffer, the cells were photographed with fluorescence microscope (Leica DMI 3000B Germany, Coumarin-channel: Ex 405 nm/Em 461 nm)

(8) This increase was determined by measuring the 12-hour area under the curve (AUC), considered a pharmacokinetic parameter that quantifies the total hormone concentration in the bloodstream over a specified period of time