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doi: 10.1093/humupd/dmaa051 62 ReinehrTWoltersBKnopCLassNHollRW

SERMs prevent estrogen's negative feedback on the HPG axis, while AIs inhibit the aromatization of testosterone to estrogens

The GSH:GSSG ratio is a rough readout of oxidative stress: A high ratio suggests resilient cells

A 20 l mix, containing 8 l of cDNA sample, 10 l of SYBR Green PCR Master Mix (Thermo Fisher Scientific), and 2 l of 10 mM forward primer and reverse primer mixture was used to performed Quantitative RT-PCR with a StepOne Plus Real-time PCR system (Applied Biosystems, Foster City, CA, United States)

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