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Switching the injection type from subcutaneous to intramuscular

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Purification and Detection High performance liquid chromatography (HPLC) purification: The synthesized polypeptides need to be purified by HPLC to remove impurities and unreacted raw materials, ensuring that the purity meets the pharmaceutic-grade standard

This is because histamine intolerance is a discrete condition with non-specific symptoms that overlap with other well-established diseases [32]