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After 1.5 h incubation at room temperature (RT), the membrane was solubilized in the buffer above supplemented with 0.5% (w/v) lauryl maltose neopentyl glycol (LMNG, Anatrace) and 0.1% (w/v) cholesterol hemisuccinate (CHS, Anatrace) for 2 h at 4 C

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Int J Environ Anal Chem 91:12271243 Beattie KA, Ressler J, Wiegand C, Krause E, Codd GA, Steinberg CEW, Pflugmacher S (2003) Comparative effects and metabolism of two microcystins and nodularin in the brine shrimp Artemia salina

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We have recently generated a transgenic mouse line that allows for tissue-selective expression of the endogenous Gcg gene [9]