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The hCTR cells were cultured in growth medium consisting of Dulbeccos Modified Eagles Medium (DMEM, Gibco, #31966-021) supplemented with 10% v/v foetal bovine serum (FBS, Gibco, #16140-071), 1% v/v Penicillin-Streptomycin (Gibco, #15140-122), 0.6 mg/mL Geneticin G418 (Gibco, #10131-027) and 0.2 mg/mL Hygromycin (Invitrogen, #10687010)

By increasing the rate of beta-oxidation, L-Carnitine provides a massive influx of acetyl-CoA to the TCA cycle

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It is also involved in regulating various bodily functions and protecting against oxidative stress

however, in vivo, one could imagine that repeated 5FU exposures would drive selection of pre-existing niches of tumours cells that had higher mitochondrial functionality