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Cell culture Clonal insulin-secreting BRIN-BD11 cells were maintained in RPMI 1640 medium containing 11.1 mM glucose, supplemented with 10% foetal bovine serum (FBS), 100 U/ml penicillin and 0.1 mg/ml streptomycin, pH 7.4, as described previously 45

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An increased ROS activates the nuclear factor E2-related factor 2 (Nrf2) transcription factor which binds to the antioxidant response element (ARE) in the promoter of the target genes to induce the expression of antioxidant genes such as NAD(P)H:quinone oxidoreductase 1, glutathione synthesis and the genes involved in mitochondrial quality and quantity control [15,16,17,18]