This method is useful to purify a protein based on their features, such as its size or specific binding affinity to a ligand
Thus, we have identified a previously unknown role of GSH in B cells: maintaining mitochondrial ETC activity
N3 bears a lactam ring, an aliphatic isobutyl group, an isopropyl group, a methyl group and an isoxazole as the side chain for the P1P5 sites, respectively
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Beads were washed 3x for 5 min each with IP wash buffer (50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1 mM EDTA, 0.05% NP-40) and eluted by boiling in 2x Laemmli sample buffer (BioRad)