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rhamnosus CCFM1228 can enhance astrocyte function in depressed mice by upregulating Gfap mRNA expression significantly

Finally, the substrate 3,3,5,5-tetramethylbenzydine (TMB) was added to the solution, which undergoes an enzymatic reaction catalyzed by HRP from which a blue color is produced that turns yellow after the addition of an acidic stop solution

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It is also a useful signal: a solution that loses its blue, turns green, or develops visible precipitate is indicating a change in the copper coordination state or the integrity of the complex